| Experiment Id | E-GEOD-65950 | Series Id | GSE65950 |
| Name | Effects of selection for ethanol preference on gene expression (RNA-Seq) in the nucleus accumbens shell of HS-CC mice | Experiment Type | RNA-Seq |
| Study Type | Baseline | Source | ArrayExpress |
| Curation Date | 2019-02-28 |
| description | Nucleus accumbens shell gene expression was assessed by RNA-Seq to determine effects of bidirectional selection for ethanol preference from a highly genetically diverse founder population. We analyzed the data with both standard methodologies to detect differential expression, and coexpression network changes, and with novel methodologies to detect alternative exon usage, and coordinated splicing pattern changes due to selection. 48 samples of HSCC animals selected for high and low ethanol preference were analyzed (selection generation 4): 24 females (12 High, 12 Low) 24 males (12 High, 12 Low). |
| notes | HSCC (heterogeneous stock-collaborative cross) mice were used for these experiments. This stock was formed by interbreeding of the eight different inbred strains used to form the Collaborative Cross: 129Sv/Im, A/J, C57BL/6, CAST/Ei, NOD/Lt, NZO/HILt, PWK/Ph and WSB/EiJ. Animals from the 4th generation selected for alcohol preference were used for these experiments. |
