Experiment Id | E-MTAB-5755 | Name | Microarrays of mouse embryonic limb skeletal muscle at days E14.5 and E18.5 lacking the RYR1 or Cav1.1 Ca2+ channels |
Experiment Type | transcription profiling by array | Study Type | WT vs. Mutant |
Source | ArrayExpress | Curation Date | 2018-11-09 |
description | Our aim is to analyze how does the lack of each of the Ca2+ channels, involved in excitation-contraction coupling in skeletal muscle - RYR1 and Cav1.1, affect gene expression in embryonic mouse limb skeletal muscle during secondary myogenesis. We extracted total RNA from the limb skeletal muscle of WT, heterozygous RYR1-/+ and Cav1.1+/-, and homozygous RYR1-/- and Cav1.1-/- mutants from 3 litters (n = 3 for each group) at days E14.5 and E18.5 and subjected it to microarray analyses. |