Experiment Id | E-MTAB-6972 | Name | Transcriptome of the liver in 15-day-old C3H/HeJ mice |
Experiment Type | RNA-Seq | Study Type | Baseline |
Source | ArrayExpress | Curation Date | 2018-08-22 |
description | Male C3H/HeOuJ mice are susceptible to spontaneous liver neoplasms. We collected liver samples from young, tumour-free mice for control experiments. Liver tissue samples were snap frozen in liquid nitrogen and total RNA was extracted using the AllPrep 96 DNA/RNA Kit (Qiagen) according to the manufacturer's instructions. Libraries were prepared using the TruSeq Stranded Total RNA Library Prep Kit with Ribo-Zero Gold (Illumina) and sequenced in an Illumna HiSeq4000 to produce 150bp paired-end reads. |