| Experiment Id | E-MTAB-7277 | Name | RNA-Seq of round spermatids comparing WT (FVBNJ) to H2A.B3 knock-out mice |
| Experiment Type | RNA-Seq | Study Type | WT vs. Mutant |
| Source | ArrayExpress | Curation Date | 2019-03-13 |
| description | The histone variant H2A.B3 (H2A.Bbd/H2A.Lap1) has a tissue-specific expression pattern and is found to be most highly expressed during spermatogenesis. In order to investigate the role of H2A.B3 in chromatin packaging and regulation of spermatogenesis we created a line of TALEN-edited FVBN mice in which a deletion was introduced into the single exon of the H2AFB3 gene. The deletion was confirmed by Sanger sequencing and off-target effects investigated using exome sequencing. The deletion is visible in the RNA-Seq data of KO mouse, and the deleterious effect on the H2A.B3 protein has been confirmed using immunohistochemistry. |
