| Experiment Id | E-MTAB-8669 | Name | Microarray of laser capture microdissected villus tip or crypt small intestinal epithelium from adult mice fed Western diet or standard diet for 4 weeks |
| Experiment Type | transcription profiling by array | Study Type | Baseline |
| Source | ArrayExpress | Curation Date | 2022-01-03 |
| description | Environmental factors, such as diet, have been described to regulate microvillar length of intestinal epithelial cells through unclear mechanisms. We fed mice a Western diet (WD) containing 40% fat, 20% fructose, and 2% cholesterol (reflective of the diet consumed by many Americans) or a Standard Diet (SD) containing 13% fat for 4 weeks. The microvillar length of villus enterocytes in the ileum was decreased by WD relative to SD. To uncover potential biological pathways regulating this response, we used laser capture microdissection to isolate RNA enriched for villus tip epithelial cells or crypt base epithelial cells from the small intestines (more specifically, the ilea) of mice fed WD or SD for 4 weeks. We then generated cDNA and performed microarray transcriptional profiling of these samples (n=4 mice per diet, with 2 sampling regions per mouse). |
