| Experiment Id | GSE151350 | Name | Diurnal gene expression in the retina of a mouse model of diabetic retinopathy |
| Experiment Type | transcription profiling by array | Study Type | WT vs. Mutant |
| Source | GEO | Curation Date | 2022-03-17 |
| description | The mammalian retina contains an endogenous circadian clock system, located in various cell types. This system enables timing of a broad range of essential retinal functions to anticipate daily changes in environmental lighting conditions. Furthermore, the circadian clocks appear to promote retinal health. A leading cause of blindness in developed countries is diabetic retinopathy. While it is clear that diabetes affects the master clock and its circadian output in the SCN, the effect of diabetic retinopathy on the retinal clock system is unknown. To investigate the influence of diabetic retinopathy on circadian regulation of the retina at a genome-wide level, microarray analysis was used to compare retinal transcriptomes between light- and dark-adapted non-diabetic and diabetic mice. Retinae of adult non-diabetic (db/+) and diabetic (db/db) mice (BKS.Cq-Dock7m +/+ Leprdb/J) were sampled at two different zeitgeber times (ZTs) during the 24-hour light/dark-cycle namely 6 hours after lights-on (ZT6) and 6 hours after lights-off (ZT18) for RNA extraction and hybridization on Affymetrix microarrays. 3 mice were used for each genotype and time-point. |
