Experiment Id | GSE168901 | Name | Single-cell Transcriptomic Analysis of the Mouse Crista Ampullaris |
Experiment Type | RNA-Seq | Study Type | Baseline |
Source | GEO | Curation Date | 2022-09-30 |
description | scRNA-seq on E18, P3 and P7 mouse crista ampullaris Cristae, including the ampullae, were dissected from E18 mice (n = 5), P3 mice (n = 8) and P7 mice (n= 12) in ice cold Hank's buffered salt solution. Pooled cristae were then dissociated by treatments with collagenase and papain. Libraries for scRNA-seq were prepared using the Chromium Single Cell 3' Library & Gel Bead Kit v3 (10x Genomics; Pleasanton, CA) per the manufacturer's instructions. Reads were aligned to mm10 and filtered using the Cell Ranger pipeline. |