| Experiment Id | GSE142851 | Name | RNA-sequencing experiments comparing P0 control and RXRG/RARb double mutants frontal cortex |
| Experiment Type | RNA-Seq | Study Type | WT vs. Mutant |
| Source | GEO | Curation Date | 2023-02-03 |
| description | To understand the functional significance of RA signaling through the RXRG/RARB heterodimer in the developing cortex, we performed RNA-seq experiments comparing different regions of the P0 mouse frontal cortex (mPFC, secondary motor cortex (MOs)/ primary motor cortex (MOp), and OFC ) in Rarb and Rxrg dKO and WT littermate controls. Freshly dissected P0 brains were frozen at -80C, and moved to RNALater 12-16 hrs prior to further dissection. Medial frontal, motor, and orbitofrontal regions were microdissected. RNA was isolated using Rneasy plus micro kit (Qiagen), and cDNA libraries were created using SMART-seq v4 Ultra Low Input Kit (Takara) and Nextera XT DNA library Prep Kit (Illumina). Libraries were normalized and sequenced at the Yale Center for Genomic Analysis (YGCA) using the NovaSeq with 100 bp paired end reads. |
