| Experiment Id | E-MNIA-69 | Name | Transcription profiling of E12.5 mouse whole embryos and placentas pooled within 3 litters to validate a new array |
| Experiment Type | transcription profiling by array | Study Type | Baseline |
| Source | ArrayExpress | Curation Date | 2022-10-23 |
| description | E12.5 mouse whole embryos and placentas were pooled within 3 litters and total RNA was extracted. Fluorescently-labeled, linearly-amplified cRNA targets were prepared from the RNA samples and compared on a novel microarray design to validate the system. A "dye-swapped" experimental design was used to assess the accuracy and precision of the system. The in situ-synthesized 60-mer oligonucleotide microarray platform contains approximately 22,000 DNA features, and was designed to detect transcripts from the entire NIA cDNA clone collection. |
