| Experiment Id | GSE112913 | Name | A network of noncoding regulatory RNAs acts in the mammalian brain III |
| Experiment Type | RNA-Seq | Study Type | WT vs. Mutant |
| Source | GEO | Curation Date | 2022-11-15 |
| description | Noncoding RNAs (ncRNAs) play increasingly appreciated gene-regulatory roles. Here, we describe a regulatory network centered on four ncRNAs--a long ncRNA, a circular RNA, and two microRNAs--using gene editing in mice to probe the molecular consequences of disrupting key components of this network. The long ncRNA Cyrano uses an extensively paired site to miR-7 to trigger destruction of this microRNA. Cyrano-directed miR-7 degradation is much more efficient than previously described examples of target-directed microRNA degradation, which come from studies of artificial and viral RNAs. By reducing miR-7 levels, Cyrano prevents repression of miR-7-targeted mRNAs and enables the accumulation of Cdr1as, a circular RNA known to regulate neuronal activity. Without Cyrano, excess miR-7 causes cytoplasmic destruction of Cdr1as, in part through enhanced slicing of Cdr1as by a second miRNA, miR-671. Thus, several types of ncRNAs can collaborate to establish a sophisticated regulatory network. miRNA expression profiling by small-RNA sequencing of cerebellum and hippocampus from wild-type (WT) and Cyrano-/- (CyrKO) mice, and of cerebellum from Cdr1as miR-671 site mutant (Cdr1as671), and double mutant (CyrKO;Cdr1as671) mice. This study consists of 18 libraries generated from 3 biological replicates per genotype for each tissue. |
