Experiment Id | GSE138234 | Name | Gtf2i and Gtf2ird1 E13.5 whole brain ChIP-seq and RNA-seq |
Experiment Type | RNA-Seq | Study Type | WT vs. Mutant |
Source | GEO | Curation Date | 2022-12-17 |
description | HetxHet breeding pairs for the Gtf2i/Gtf2ird1 double mutants and Gtf2ird1 were set up for timed breedings. E13.5 embyros of WT, HET, and HOM mutants (n=3 for each genotype and each cross), were used for RNA-seq. Similar breedings were done for ChIP-seq. The ChIP-seq WT controls were E13.5 embyros from WT FVB/ANTJ x FVB/ANTJ and compared to HOM Gtf2i/Gtf2ird1 double mutants and HOM Gtf2ird1 single mutants. There were n=3 WT and n=3 hom Gtf2ird1 single mutants for Gtf2ird1 ChIP-seq. There were n=4 WT and n=4 hom Gtf2i/Gtf2ird1 double mutants for Gtf2i ChIP-seq. Each genotype had the sample matched input control along with ChIP sample. Gtf2i and Gtf2ird1 E13.5 ChIP-seq and RNA-seq |