| Experiment Id | GSE155428 | Name | Expression data (mRNA) from GD-9.5 (E9.5), Folr1+/+ (Folr1 WT) and Folr-/- (Folr1 KO) mouse embryos |
| Experiment Type | transcription profiling by array | Study Type | WT vs. Mutant |
| Source | GEO | Curation Date | 2023-02-14 |
| description | Folic acid is a nutrient essential for embryonic development. Folate deficiency can cause embryolethality or neural tube defects and orofacial anomalies. Folate receptor 1 (Folr1), is a folate binding protein that facilitates the cellular uptake of dietary folate. Animal studies highlighted critical roles of folate in embryogenesis including orofacial development. Orofacial clefts were observed in the offspring of experimental animals on folic acid-deficient diets. Mice lacking Folr1 (the gene encoding folate receptor 1) also demonstrated a variety of orofacial defects. In order to investigate potential roles of Folr1 during embryogenesis and to identify various genes functionally associated with this receptor, total RNA preparations from gestation day (GD)-9.5, Folr1+/+ and Folr1-/- mouse embryos were used to hybridize messenger RNA arrays, to identify genes expressed in wild type (Folr1+/+) and knockout (Folr1-/-) embryos. We used total RNA preparations from gestation day (GD)-9.5 Folr1+/+ (wild type) mouse embryos (3x biological replicates/samples) and Folr1-/- (knockout) mouse embryos (2x biological replicates/samples) to perform global gene (mRNA) expression profiling analyses. For messenger RNA profiling we used Affymetrix GeneChip Mouse Genome 430A arrays. One of the objectives of this study is to identify and compare the expression of constitutively and differentially expressed genes between Folr1+/+ (Wild type) and Folr1-/- (knockout) mouse embryos. |
