| Experiment Id | GSE172167 | Name | A Single Cell Atlas of Spared Tissue After Spinal Cord Injury Reveals the Mechanisms of Restricted Repair |
| Experiment Type | RNA-Seq | Study Type | Baseline |
| Source | GEO | Curation Date | 2023-07-07 |
| description | Trauma to the nervous system elicits multi-cellular responses at the site of direct injury, in nearby tissue, and throughout the entire body. In spinal cord injury, the "spared" tissue below the lesion is the target of promising therapies. However, its cellular and molecular changes are not well understood, hampering efforts to promote recovery. Here, we used single nucleus sequencing to create an atlas of the lumbar spinal cord after thoracic contusion injury and found unexpected signs of latent potential for tissue repair. Amongst neurons, rare cell types induced a molecular signature of regeneration. And amongst microglia, we identified a population of "trauma associated microglia" that were present in the white matter near degenerating axons. Viral over-expression of the Igf1/Spp1 trophic factors expressed by these cells expanded the population and promoted the clearance of myelin debris. These findings illuminate endogenous mechanisms of spared tissue repair as candidates for targeted therapy. 5 conditions (uninjured, 1 day post injury, 1 week post injury, 3 weeks post injury and 6 weeks post injury) with 3 biological replicates each, totaling 15 lumbar spinal cords that were taken through snRNAseq and downstream analysis. |
