| Experiment Id | GSE207736 | Name | Single-nucleus RNA sequencing of the mouse hypothalamus in the fed and fasted state |
| Experiment Type | RNA-Seq | Study Type | Baseline |
| Source | GEO | Curation Date | 2023-07-07 |
| description | To provide a summary of cells within the mouse hypothalamus in the fed and fasted state, we performed single nucleus RNA sequencing on 12 mice fed ad libitum, and 6 mice that were fasted overnight. Hypothalami were dissected and stored at -80ºC overnight. The next day single nucleus supensions were made, pooling together hypothalamic from the same feeding condition (2 x ad libitum prepared on separate days; 1 x overnight fast). Susepensions were FACS sorted for DRAQ5 positive events. Single nucleus RNA sequencing libraries were generated using the 10X Chromium single cell 3' reagents. The hypothalamus was dissected from 12 adlib fed (2 cohorts) and 6 overnight (16 hr) fasted mice, and a single nucleus suspension was created using a dounce homogenizer and purified using FACS, sorting for DRAQ5 positive events. These samples (2 adlib fed and 1 overnight fasted) were submitted for single nucleus RNA sequencing |
