| Experiment Id | GSE99551 | Name | Gene expression analysis of WT and Adam19KO cardiac neural crest cells |
| Experiment Type | transcription profiling by array | Study Type | WT vs. Mutant |
| Source | GEO | Curation Date | 2023-12-07 |
| description | Neural crest cells (NCCs) migrate into the heart, and their defects lead to congenital heart diseases. Despite the importance of cardiac NCCs (CNCCs), their final fates and underlying mechanisms of the fate specification have been unknown. Here we report that CNCCs differentiate from bipotent progenitors to tenocytes by preventing chondrogenesis. CNCCs highly express a disintegrin and metalloprotease 19 (Adam19) after migrating into the heart. Adam19-deleted CNCCs fail to suppress expression of Sox9 and generate abnormal cartilage, while they normally form the tendon connecting the aorta and pulmonary artery. Highly expressed Sox9 in CNCCs is necessary and sufficient for their chondrogenic fate decision. Adam19 inhibits response to BMP, preventing CNCCs from enhanced Sox9 expression. Our study reveals a novel fate of the NCC, and moreover, provides insights into how postmigratory NCCs define their fates locally. E14.5 Cardiac neural crest cells (CNCCs) were isolated from Adam19+/+; Wnt1-Cre; R26tdTomato/+ and Adam19-/-; Wnt1-Cre; R26tdTomato/+ embryonic hearts using FACS. Total RNA of sorted CNCCs were extracted with RNeasy micro Plus Kit(Qiagen). Mouse Gene 1.0 ST Array(Affymetrix) was used to compare Adam19+/+ CNCCs and Adam19-/- CNCCs. |
