| Experiment Id | GSE79410 | Series Id | E-GEOD-79410 |
| Name | Transforming growth factor-beta and Notch ligands act as opposing environmental cues in the plasticity of type 3 innate lymphoid cells | Experiment Type | RNA-Seq |
| Study Type | Baseline | Source | GEO |
| Curation Date | 2024-01-25 |
| description | Group 3 innate lymphoid cells (ILC3) are composed of NCR- and NCR+ subsets located at mucosal sites exposed to billions of commensal microbes and potentially harmful pathogens. Together with T cells, the various ILC3 subsets maintain the balance between homeostasis and immune activation. Using genetic mapping, we reveal here the existence of a new subset of NCR- ILC3 transiently expressing Ncr1 but strongly related to unlabeled NCR- ILC3, demonstrating previously unsuspected heterogeneity within the NCR- ILC3 population. Notch signaling is required for the differentiation of NCR- ILC3 into NCR+ ILC3. However, we show here that Notch signaling must be sustained for the maintenance of the NCR+ phenotype and that TGF-beta impairs the development of NCR+ ILC3. Thus, ILC3 diversity and the plasticity of the NCR- and NCR+ subsets is regulated by the balance between the opposing effects of Notch and TGFbeta signaling, maintaining homeostasis in the face of continual challenges. Transcriptional profiling of three ILC subsets (NCR-FM-, NCR-FM- and NCR+FM+) using RNA sequencing |
