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HT Experiment :

Experiment Id  GSE77374 Series Id  E-GEOD-77374
Name  RNA-seq data from murine eGFP+ relbfl/flnfkb2fl/flCg1-Cre and Cg1-Cre splenic germinal center B cells purified by fluorescent activated cell sorting. Experiment Type  RNA-Seq
Study Type  WT vs. Mutant Source  GEO
Curation Date  2024-01-24
description  RNA-seq analysis of murine eGFP+ relbfl/flnfkb2fl/flCg1-Cre and Cg1-Cre splenic germinal center B cells identifies genes regulated by the transcription factors RELB and p52 (NF-kB2) in germinal center B cells. Germinal center B cells from 12-week old relbfl/flnfkb2fl/flCg1-Cre and Cg1-Cre littermate mice immunized with sheep red blood cells (SRBC) were isolated at day 7 after immunization by flow cytometric sorting from splenic mononuclear cells. RNA was isolated, amplified and submitted for RNA-sequencing on an Illumina HiSeq2500 instrument for 35-40 million 2x50 paired-ended reads.
  • variables:
  • bulk RNA-seq,
  • genotype

1 Publications

Trail: HTExperiment

5 Samples

Trail: HTExperiment