| Experiment Id | GSE75920 | Series Id | E-GEOD-75920 |
| Name | The major diversification of Vgamma1.1+ and Vgamma2+ thymocytes in mice occurs after commitment to the gamma-delta T-cell lineage | Experiment Type | transcription profiling by array |
| Study Type | Baseline | Source | GEO |
| Curation Date | 2024-04-12 |
| description | Adult murine gamma-delta thymocytes can be divided into two major subsets based on their expression of TCRVgamma1.1 or TCRVgamma2, respectively. gamma-delta thymocytes mature through distinct stages characterized by CD24 and CD73 in the following order: immature uncommitted (CD24+CD73-), immature committed (CD24+CD73+) and mature (CD24-CD73+). In this dataset we have sorted gamma-delta thymocytes into 6 distinct populations based on subset and developmental stage and analyzed their gene expression. These data were used to identify 3400 differentially expressed genes during gamma-delta thymocyte development and to show that the two major adult gamma-delta T cells follow a similar path through early development and mainly diversify after commitment to the gamma-delta T cell lineage. 22 samples (6 populations 3-4 biological replicates) were analyzed for differential expression to identify genes shared between cells of the different subsets at each developmental stage. |
