| Experiment Id | GSE192999 | Name | Spatially resolved single-cell deconvolution of bulk transcriptomes using Bulk2Space |
| Experiment Type | RNA-Seq | Study Type | Baseline |
| Source | GEO | Curation Date | 2024-04-22 |
| description | We developed a spatially resolved RNA-seq protocol based on laser capture microdissection (LCM) and spatial barcoding strategy termed Spatial-seq. Here, we provide a demo transcriptomic data derived from mouse hypothalamus for an application of our newly proposed spatial deconvolution algorithm termed Bulk2Space. We want to reveal the spatial heterogeneity and cellular diversity within the bulk brain region. We performed laser capture microdissection to isolate the hypothalamus region from mouse brain sections at sagittal direction. The brain sections were first registered to the Allen Brain Atlas, and each main region was annotated. The annotated region outline was then imported into LCM. The hypothalamus region was cut by LCM and followed with RNA-seq to obtain the gene expression matrix. |
