| Experiment Id | GSE165274 | Name | Characterization of novel whole body Poldip2 knockout mice generated by [Precisely "flox"ing Poldip2 gene] and crossing with Cre deleter mice |
| Experiment Type | RNA-Seq | Study Type | WT vs. Mutant |
| Source | GEO | Curation Date | 2024-05-03 |
| description | Poldip2 is a multifunctional protein whose roles are only partially understood. The previous Poldip2 knockout mouse generated by the gene trap model suffers from two limitations: perinatal lethality in homozygotes and constitutive Poldip2 inactivation. To overcome these limitations, we developed a new conditional floxed Poldip2 mouse. Here, we correctly targeted floxed Poldip2 mice to produce a new constitutive knockout line by crossing with a Cre deleter. In contrast to the gene trap model, many homozygous knockout mice were viable, in spite of having no Poldip2 expression. To characterize the effect of Poldip2 ablation in the vasculature, we performed a RNA-seq study using the carotid arteries of these mice. The carotid arteries were harvested from the Poldip2 knockout mice (n=4) as well as from the littermate controls (n=4). The lumen of these areries were quickly flushed with Qiazol to extract the endothelial-enriched RNAs. Also, RNAs were extracted from the leftover arteries (containing the media and adventitia, consisting of smooth muscle cells and other adventiatial cells). These RNAs were subjected to RNAseq to determine the effect of Poldip2 knockdown in endothelium and in the vascular smooth muscle fraction of the carotid arteries. |
