| Experiment Id | E-GEOD-2205 | Series Id | GSE2205 |
| Name | Transcription profiling of mouse model or ERM deficiency - analysis of germ stem cell loss | Experiment Type | transcription profiling by array |
| Study Type | WT vs. Mutant | Source | ArrayExpress |
| Curation Date | 2024-07-09 |
| description | ERM knockout mice were produced. Testis develop a Sertoli cell only; syndrome, which appears complete by 10 weeks of age in male ERM knockout mice. Three sets of Affymetrix comparisons were made. #1 and #2, Wt or ERM knockout testis from 4 week old males, totaly RNA. This time preceeds any significant histologic difference between the wt or; knockout. The results was the finding that only a very specific set or; markers for spermatogonial STEM CELLs were found to be greatly reduced in the ERM knockout. #3 and #4. The sertoli cell line TM4 was transduced with a retroviral; ERM. The wild type TM4 cell line was compared to this TM$-ERM cell line,; with the finding that only a few genes were increased in the TM4-ERM line. #5, #6 and #7. Primary Sertoli cells were purified from 4 week old wild; type or ERM knockout or from 10 week old ERM knockout mice and compared. |
