| Experiment Id | GSE195459 | Name | Photo-isolation chemistry for high-resolution and deep spatial transcriptome with tissue sections |
| Experiment Type | RNA-Seq | Study Type | Baseline |
| Source | GEO | Curation Date | 2024-02-14 |
| description | We established a high-depth spatial transcriptomics technology, photo-isolation chemistry (PIC; Honda, et al., Nat Commun, 2021), which is able to isolate gene expression profiles only from photo-irradiated region out of whole tissues. In the original protocol, only the fresh-frozen sections were available, but we have now optimized it applicable to formalin-fixed frozen and formalin-fixed paraffin sections. Here, we provide an updated protocol of PIC. We performed PIC analysis to isolate expression profiles of hippocampal regions [CA1, CA2 and dentate gyrus (DG)] from fresh frozen, formalin-fixed frozen and formalin-fixed paraffin sections of mouse brain. |
