| Experiment Id | GSE83146 | Series Id | E-GEOD-83146 |
| Name | Single-Cell RNAseq Reveals That Pancreatic beta-Cells From Very Old Male Mice Have a Young Gene Signature | Experiment Type | RNA-Seq |
| Study Type | Baseline | Source | GEO |
| Curation Date | 2024-01-25 |
| description | Aging improves pancreatic beta-cell function in mice. This is a surprising finding since aging is typically associated with functional decline. We performed single-cell RNA sequencing of β-cells from 3 and 26 month old mice to explore how changes in gene expression contribute to improved function with age. The old mice were healthy, had reduced blood glucose levels and increased beta-cell mass, which correlated to their body weight. beta-cells from young and old mice had similar transcriptome profiles. In fact, only 193 genes (0.89% of all detected genes) were significantly regulated (>= 2-fold; false discovery rate < 0.01; normalized counts > 5). Of these, 183 were downregulated and mainly associated with pathways regulating gene expression, cell cycle, cell death and survival as well as cellular movement, function and maintenance. Collectively, our data show that beta-cells from very old mice have transcriptome profiles similar to those of young mice. These data support previous findings that aging is not associated with reduced beta-cell mass or functional beta-cell decline in mice. Single-cell RNA sequencing of mouse pancreatic islet beta cells |
