| Experiment Id | GSE134181 | Name | TrxG complex catalytic and non-catalytic activity play distinct roles in pancreas progenitor specification and differentiation (E18.5 Dpy30deltaP pancreas scRNA-seq) |
| Experiment Type | RNA-Seq | Study Type | WT vs. Mutant |
| Source | GEO | Curation Date | 2022-12-15 |
| description | Purpose: To gain insight into why Dpy30deltaP acinar cells fail to express terminal makers, we performed scRNA-seq using E18.5 control and Dpy30deltaP pancreata. Methods: Floxed Dpy30 mice were crossed to Pdx1-Cre driver mice to obtain conditional deletion of Dpy30 exon 4 in the pancreas. In all studies, knockout mice (Dpy30deltaP, Pdx1-Cre; Dpy30flox/flox) were compared to littermate controls (Dpy30flox/flox or Dpy30flox/wt). Results: In total, we sequenced 8,050 control and 7,858 Dpy30deltaP cells that with the E13.5 scRNA-seq data allowed us to identify 2,207 control and 2,354 Dpy30deltaP epithelial cells. We found a cluster that contained almost entirely Dpy30deltaP cells, where the expression of acinar cell digestive enzymes was consistently low. The distribution of gene expression values for terminal acinar markers suggested an increased distribution in gene expression levels in the Dpy30deltaP cells relative to control cells; however, this was not the case for terminal endocrine markers. Comparing the variance-to-mean ratios in the Dpy30deltaP and control cells showed an increase in variance in the Dpy30deltaP acinar cells but not in endocrine cells. Conclusions: Overall, these data suggest increased variation in acinar cell transcript expression in the Dpy30deltaP pancreas. Single-cell RNA-sequencing was performed using one E18.5 control and one E18.5 Dpy30deltaP pancreas |
