| Experiment Id | GSE150150 | Name | Transcriptional landscape of fate choices in the sensory lineages |
| Experiment Type | RNA-Seq | Study Type | Baseline |
| Source | GEO | Curation Date | 2023-02-10 |
| description | Somatic sensation is defined by the existence of a diversity of primary sensory neurons with unique biological features and response profiles to external and internal stimuli. However, there is no coherent picture about how this diversity of cell states is transcriptionally generated. Here, we used deep single cell analysis to resolve fate splits and molecular biasing processes during sensory neurogenesis. Our results revealed a complex series of successive and specific transcriptional changes in post-mitotic neurons that delineate hierarchical regulatory states leading to the generation of the main sensory neuron classes. In addition, our analysis identified previously undetected early gene modules expressed long before fate determination although being clearly associated with the final sensory subtypes. Overall, fate choice in sensory neurons involves initial co-activation of counteracting regulatory determinants of alternative lineages priming intermediate post-mitotic neuronal cells for a binary cell fate choice. Hence, the diversity of sensory neurons is generated through successive bi-potential intermediates in which synchronization of relevant gene modules and concurrent repression of competing fate programs precede cell fate stabilization and final commitment. Single cells were collected at different locations on the embryo using different tracings: Wnt1 traced cells were collected in the trunk at E9.5 and E10.5. Plp1 traced cells were collected in cranio/cervico/thoracic region at E12.5. Il1 traced cells were collected in the DRGs at E9.5 and E10.5. TrkC traced cells were collected in the DRGs at E11.5 and E12.5. |
