| Experiment Id | GSE162103 | Name | PDGFRbeta+ cells play a dual role as hematopoietic precursors and niche cells during mouse ontogeny |
| Experiment Type | RNA-Seq | Study Type | WT vs. Mutant |
| Source | GEO | Curation Date | 2023-10-10 |
| description | Hematopoietic stem cell (HSC) generation in the aorta-gonads-mesonephros region requires HSC specification signals from the surrounding microenvironment. In zebrafish, PDGF-B/PDGFRbeta signaling controls hematopoietic stem/progenitor cell (HSPC) generation and is required in the HSC specification niche. Little is known about murine HSPC specification in vivo and whether PDGF-B/PDGFRbeta is involved. Here we show that PDGFRbeta is expressed in distinct perivascular stromal cell layers surrounding the mid-gestation dorsal aorta, and its deletion impairs hematopoiesis. We demonstrate that PDGFRbeta+ cells play a dual role in murine hematopoiesis. They act in the aortic niche to support HSPCs, and in addition, PDGFRbeta+ embryonic precursors give rise to a subset of HSPCs that persist into adulthood. These findings provide crucial information for the controlled production of these clinically important cells in vitro. Single-cell transcriptome profiling of E11 AGM samples from PDGFRB +/+ (WT) (n=1) and PDGFRB -/- (KO) (n=1) mice |
