Experiment Id | GSE172460 | Name | Single nucleus RNA-seq atlas of mouse Lepr-expressing hypothalamic cells |
Experiment Type | RNA-Seq | Study Type | Baseline |
Source | GEO | Curation Date | 2024-04-24 |
description | To uncover the cellular identifies of the mouse hypothalamic Lepr cells, we used single nucleus RNA-seq (snRNA-seq) from enriched hypothalamic Lepr cells. Lepr-Cre;R26-LSL-Sun1-sfGFP mice were maintained on ad libitum chow prior to microdissection of the hypothalamus. We then isolated nuclei, FACS sorted for GFP-positive nuclei, and subjected these nuclei to snRNA-seq using the 10x Genomics 3' gene expression platform. |