| Experiment Id | GSE97367 | Name | BioProject PRJNA381064: Convergent origination of a Drosophila-like dosage compensation mechanism in a reptile lineage (Gene expression profiling in several tetrapod species, bulk tissue RNA-seq) |
| Experiment Type | RNA-Seq | Study Type | Baseline |
| Source | GEO | Curation Date | 2023-07-14 |
| description | In order to study the green anole dosage compensation mechanism we generated strand-specific RNA-seq libraries for a total of 186 samples from the green anole and other representative amniotes (human, mouse, opossum, platypus, chicken and xenopus). Moreover, in order to understand the dosage compensation mechanism of A. carolinensis, we generated ChIP-Seq data for the histone modification H4K16ac and compared levels of acetylation between males and females. Moreover, we reconstructed 7 Y-linked transcripts of Anolis carolinensis based on a male/female subtraction approach and validate these Y sequences using re-sequenced genomes. We generated strand-specific RNA-seq libraries using the Illumina TruSeq Stranded mRNA Library protocol. Sample size (minimum one male and one female for each species) was established to have a wide spectrum across amniotes and to capture general male/female sex-specific patterns. Each source RNA was of high quality, as assessed using a Fragment Analyzer machine from Advanced Analytical (RIN median = 9, RQN median = 8). Each library was sequenced on Illumina HiSeq 2500 platforms at the Lausanne Genomic Technologies Facility (https://www.unil.ch/gtf/en/home.html). At least 17 million sequencing reads (100 nt, single-end) were produced for each library (median: 34 million reads). Examination of H4K16ac enrichment between males and females in liver and brain. Two biological replicates, four genomic DNA input libraries. Y-linked transcripts of Anolis carolinensis were obtained using a male/female subtraction approach. The sequences were validated using re-sequenced male and female genomes. |
