| Experiment Id | GSE80430 | Name | Identification of a novel PPARbeta/delta/miR-21-3p axis in UV-induced skin inflammation [human miRNA-seq] |
| Experiment Type | RNA-Seq | Study Type | WT vs. Mutant |
| Source | GEO | Curation Date | 2024-04-15 |
| description | Although excessive exposure to UV is widely recognized as a major factor leading to skin perturbations and cancer, the complex mechanisms underlying inflammatory skin disorders resulting from UV exposure remain incompletely characterized. The nuclear hormone receptor PPARbeta/delta is known to control cutaneous repair and UV-induced cancer development. Here, we describe a novel PPARbeta/delta-dependent molecular cascade involving TGFbeta-1 and miR-21-3p, which is activated in the epidermis in response to UV exposure. We establish that the passenger miRNA miR-21-3p, that we identify as a novel UV-induced miRNA in the epidermis, plays a pro-inflammatory function in keratinocytes, and that its high level of expression in human skin is associated with psoriasis and squamous cell carcinomas. Finally, we provide evidence that inhibition of miR-21-3p reduces UV-induced cutaneous inflammation in ex vivo human skin biopsies, thereby underlining the clinical relevance of miRNA-based topical therapies for cutaneous disorders. Epidermis and dermis, PPARB KO vs WT, 3-4 biological replicates per condition |
