| Experiment Id | GSE85422 | Series Id | E-GEOD-85422 |
| Name | Gene expression analysis of CD122HI versus CD122LO Vgamma7+ murine small intestinal IEL at 14-17 days of age | Experiment Type | RNA-Seq |
| Study Type | Baseline | Source | GEO |
| Curation Date | 2024-01-25 |
| description | Background: The neonatal (D14-17) murine small intestinal epithelium is enriched for Vgamma7+ IEL displaying an 'immature' CD122LO cell surface phenotype. We believe these cells are precursors for signature CD122HI Vgamma7+ IEL that predominate among small intestinal IEL from postnatal D21. Method: To further characterise this potential precursor-product relationship, CD122hi Vg7+ and CD122lo Vg7+ IEL were purified from individual D14-D17 mice on four independent occasions and compared by total RNAseq. Conclusion: ~3000 genes are differentially expressed between CD122HI and CD122LO murine Vg7+ neonatal IEL 4 independent Paired samples of CD122hi Vg7+ and CD122lo Vg7+ IEL were purified from the small intestinal epithelium of pooled 14-17 day old mice. RNA was isolated from sorted samples. Gene expression analysis was performed by total RNAseq. |
