| Experiment Id | GSE213059 | Name | Losing the maternal effect gene Nlrp2 alters the ovulated mouse oocytes transcriptome and impacts histone demethylase KDM1B expression |
| Experiment Type | RNA-Seq | Study Type | WT vs. Mutant |
| Source | GEO | Curation Date | 2023-07-10 |
| description | We performed RNA sequencing on pools of oocytes after inducing ovarian stimulation in wild-type and Nlrp2-null female mice. We found 231 differentially expressed genes (DEGs) in Nlrp2-null COCs compared to WT COCs (123 up- and 108 downregulated; adjusted p<0.05). The DEGs we identified were enriched for processes involved in neurogenesis, gland morphogenesis, and protein metabolism and for post-translationally methylated proteins. When we compared our RNA sequencing results to an oocyte-specific reference transcriptome that contains many previously unannotated transcripts, we found 228 DEGs, including genes not identified with the first analysis. Intriguingly, 68% and 56% of DEGs from the first and second analyses, respectively, overlap with oocyte-specific hyper- and hypomethylated domains. Transcriptome profiling of Nlrp2-null and WT oocytes |
