| Experiment Id | GSE270328 | Name | Anterior and Posterior regions of mouse embryos with combinatory mutations of Fuz and Gpr161 |
| Experiment Type | RNA-Seq | Study Type | WT vs. Mutant |
| Source | GEO | Curation Date | 2024-10-29 |
| description | Gpr161 and Fuz are Shh signaling molecules, and both null mutations are associated with neural tube defects in mice. As their genetic relationship in Shh signaling during neural tube development is unknown, we generated double homozygous null mutant mice for phenotyping and associated molecular changes. We observed the rescued NTD phenotypes in double homozygous null mutant embryos compared to those in single Gpr161 null mutant embryos. However, the range of rescued phenotypes is distinct between anterior and posterior NTDs. Therefore, we sought to identify the comparison in gene expression between the anterior and posterior regions of each genotype embryos and between each genotype. We generated the samples for the anterior regions (above third branchial arches) and the posterior regions (below forelimbs) of WT, Gpr161 knock out (KO), Fuz KO, and double KO embryos at E10.5 to compare gene expression patterns of each genotyped embryo depending on the regions. *************************************************************** Submitter states that missing raw files are due to file loss. *************************************************************** |
