| Experiment Id | GSE172202 | Name | TRAP-seq of VMH Lepr-Slc17a6 neurons |
| Experiment Type | RNA-Seq | Study Type | Baseline |
| Source | GEO | Curation Date | 2024-12-19 |
| description | To identify transcripts enriched in ventomedial hypothalamus (VMH) neurons that contain both Lepr and Slc17a6, we used translating affinity ribosome purification combined with RNA-seq (TRAP-seq) from Lepr-Cre;Slc17a6-Flpo mice. Lepr-Cre;Slc17a6-Flpo mice were crossed to a ROSA26 Cre- and Flp-dependent EGFP-L10a line. The VMH was microdissected and ribosomes were isolated using an anti-GFP-coated beads. RNA was isolated from the beads and the supernatant and converted to cDNA. Illumina libraries were prepared and sequenced on an Illumina HiSeq machine for 52 cycles. |
