| Experiment Id | GSE219214 | Name | Impact of Vitamin-D3 on the gene expression profile of mouse colon |
| Experiment Type | RNA-Seq | Study Type | WT vs. Mutant |
| Source | GEO | Curation Date | 2024-12-20 |
| description | Vitamin-D3 (VitD3) exhibits pleiotropic effects in hosts physiology by acting on several organs. To mechanistically understand how VitD3 influences the colon physiology we interrogated the gene expression profile of mouse colon in response to high or low VitD3 bioavailability. VitD3 tissue bioavailability was modulated in mice with the use of diets supplemented with different concentrations of VitD3 (0, 2 or 10 IU/g) and the use of Gc knockout mouse strain in where VitD3 bioavailability is enhanced. Vitamin-D3 (VitD3) exhibits pleiotropic effects in hosts physiology by acting on several organs. To mechanistically understand how VitD3 influences the colon physiology we interrogated the gene expression profile of mouse colon in response to high or low VitD3 bioavailability. VitD3 tissue bioavailability was modulated in mice with the use of diets supplemented with different concentrations of VitD3 (0, 2 or 10 IU/g) and the use of Gc knockout mouse strain in where VitD3 bioavailability is enhanced. RNA will be extracted from 5 biological replicates (5 individual mice) per condition (20 samples in total). Male mice (8 weeks old) were fed with the following diets 3 weeks :1)C57BL/6J/WT mice that were fed with standard VitD3 diet (2 IU/g) for 3 weeks (n=5), 2) C57BL/6J/WT mice that were fed with high VitD3 diet (10 IU/g) for 3 weeks (n=5), 3) Gc KO mice that were fed with standard VitD3 diet (2 IU/g) for 3 weeks (n=5), 4) Gc KO mice that were fed with VitD3 deficient diet (10 IU/g) for 3 weeks (n=5) |
