| Experiment Id | GSE161877 | Name | Analysis of differential gene expression in the choroid plexus (ChP) of P60 mice at 9am and 9pm |
| Experiment Type | RNA-Seq | Study Type | Baseline |
| Source | GEO | Curation Date | 2025-01-09 |
| description | 3 mice per timepoint (9am and 9pm) Cerebrospinal fluid (CSF) provides vital support for the central nervous system (CNS). Abnormal CSF components have been associated with a number of disease states including conditions with disrupted circadian rhythmicity like Alzheimer's disease and bipolar disorder. The choroid plexus (ChP) is specialized to secrete CSF and displays circadian rhythmicity, but how the output of this tissue changes thorough the day to alter the CNS environment is unknown. In other tissues, protein synthesis rates often vary across time of day. Using ribosomal profiling, we identified translation and metabolism as downstream of the circadian clock in the ChP. Further, we identify ChP secreted factors that are differentially regulated by translation and dependent on core circadian machinery and feeding for this differential expression. These results provide insight into changing ChP circadian output that could dramatically change the CNS environment throughout the day. TRAP mRNAs were isolated and sequenced from the choroid plexus (ChP) of P60 mice |
