| Experiment Id | GSE244392 | Name | A sexually dimorphic hepatic cycle of very low density lipoprotein uptake and assembly |
| Experiment Type | RNA-Seq | Study Type | Baseline |
| Source | GEO | Curation Date | 2025-02-05 |
| description | Single-cell transcriptomes can reveal spatiotemporal programmes of liver function on the sublobular scale. However, how sexual dimorphism affects this space-time logic remains poorly understood. To address this, we performed single-cell RNA-seq in the mouse liver, which allowed us to model how lobular position, circadian time and sex shape the transcriptome. Mice were perfused with collagenase via the inferior vena cava, livers isolated, and cells released into medium and enriched for hepatocytes with a centrifugation step. The cells were then frozen. Upon thawing, sequencing libraries for scRNA-seq were constructed using 10x Genomics Chromium v3. |
