| Experiment Id | GSE279295 | Name | Impact of mitochondrial citrate carrier expression on metabolism in the developing heart |
| Experiment Type | transcription profiling by array | Study Type | WT vs. Mutant |
| Source | GEO | Curation Date | 2025-02-05 |
| description | Slc25a1 encodes for the mitochondrial citrate carrier, a mitochondrial inner membrane transporter that mediates mitochondrial citrate export. Systemic deletion of Slc25a1 (both homozygous and heterozygous loss) leads to cardiac structural defects and mitochondrial dysfunction. Transcriptomic profiles of metabolic gene expression in the the developing mouse heart at E17.5 reveal alterations in metabolic pathways including oxdiative phosphorylation, supporting mitochondrial structural and functional defects observed with loss of this transporter. In this study embryonic mouse hearts were collected from timed matings of Slc25a1 heterozgyous knockout mice at E17.5. Hearts were collected from wild type (WT), Slc25a1 heterozygous knockout (HET) and Slc25a1 homozygous knockout (KO) embryos. Total RNA was extracted from each heart and analysed using the NanoString nCounter platform to examine the expression of metabolic genes. 3 groups: WT, HET and KO. 4 samples per group. |
