Experiment Id | GSE274497 | Name | Spatial transcriptomics of murine eyelid |
Experiment Type | RNA-Seq | Study Type | Baseline |
Source | GEO | Curation Date | 2025-02-14 |
description | Meibomian glands (MGs) are specialized holocrine sebaceous glands embedded in the tarsal plate of the eyelids. The cell heterogeneity of MGs is not well characterized. In this study, we performed a spatial transcriptomic analysis of murine eyelid using the 10X Visium platform. A pair of upper and lower eyelids from an 8-week-old male C57BL/6J mouse was dissected, sectioned at 10 m, and mounted onto the assay slide containing four capture areas (6.5mm x 6.5mm) with spatially barcoded poly T capture probes. The subsequent procedures were conducted using the 10x Visium Spatial Gene Expression kit, according to the manufacturers instructions. |