| First Author | Shi P | Year | 2023 |
| Journal | Oral Dis | Volume | 29 |
| Issue | 8 | Pages | 3551-3558 |
| PubMed ID | 36520568 | Mgi Jnum | J:377669 |
| Mgi Id | MGI:8273761 | Doi | 10.1111/odi.14472 |
| Citation | Shi P, et al. (2023) Activation of Wnt signaling in Axin2(+) cells leads to osteodentin formation and cementum overgrowth. Oral Dis 29(8):3551-3558 |
| abstractText | OBJECTIVES: In this study, we used the mouse incisor model to investigate the regulatory mechanisms of Wnt/beta-catenin signaling on Axin2(+) cells in tooth development. MATERIALS AND METHODS: Axin2(lacZ/+) reporter mice were used to define the expression pattern of Axin2 in mouse incisors. We traced the fate of Axin2(+) cells from postnatal Day 21 (P21) to P56 using Axin2(CreERT2/+) and R26R(tdTomato/+) reporter mice. For constitutive activation of Wnt signaling, Axin2(CreERT2/+) , beta-catenin(flox(Ex3)/+) , and R26R(tdTomato/+) (CA-beta-cat) mice were generated to investigate the gain of function (GOF) of beta-catenin in mouse incisor growth. RESULTS: The X-gal staining of Axin2(lacZ/+) reporter mice and lineage tracing showed that Axin2 was widely expressed in dental mesenchyme of mouse incisors, and Axin2(+) cells were essential cell sources for odontoblasts, pulp cells, and periodontal ligament cells. The constitutive activation of Wnt signaling in Axin2(+) cells resulted in the formation of osteodentin featured with increased DMP1 and dispersed DSP expression and overgrowth of cementum. CONCLUSION: Wnt signaling plays a key role in the differentiation and maturation of Axin2(+) cells in mouse incisors. |
