| First Author | Wang DM | Year | 2026 |
| Journal | STAR Protoc | Volume | 7 |
| Issue | 2 | Pages | 104551 |
| PubMed ID | 42105238 | Mgi Jnum | J:390299 |
| Mgi Id | MGI:8395211 | Doi | 10.1016/j.xpro.2026.104551 |
| Citation | Wang DM, et al. (2026) Protocol to generate endothelial cell-specific knockout mouse models using Cas9/Cdh5-Cre mice coupled with sgRNA. STAR Protoc 7(2):104551 |
| abstractText | The vascular endothelium is a critical regulator of vascular homeostasis and tissue fluid balance, and mouse models are essential for studying these processes in vivo. Here, we present a protocol to generate adult endothelial cell (EC)-specific gene knockout (KO) mouse models. We describe steps for Cas9-active and Cdh5-Cre-positive (Cas9/Cdh5-Cre) mouse line generation, single guide RNA (sgRNA) design for vector construction, plasmid DNA generation, and liposome preparation. We then detail procedures for liposome/plasmid complex injection, lung harvest, homogenization, protein quantification, and verification with western blotting. For complete details on the use and execution of this protocol, please refer to Wang et al.(1). |
