| First Author | Li L | Year | 2023 |
| Journal | STAR Protoc | Volume | 4 |
| Issue | 4 | Pages | 102595 |
| PubMed ID | 37740915 | Mgi Jnum | J:375644 |
| Mgi Id | MGI:7643286 | Doi | 10.1016/j.xpro.2023.102595 |
| Citation | Li L, et al. (2023) Sample enrichment for single-nucleus sequencing using concanavalin A-conjugated magnetic beads. STAR Protoc 4(4):102595 |
| abstractText | Single-cell/nucleus sequencing has been increasingly used to study specific cell populations. However, cells/nuclei often become diluted during isolation steps and are difficult to reconcentrate through centrifugation. Here, we present a protocol for sample enrichment using concanavalin A-conjugated magnetic beads. We describe steps for dissection, nuclei isolation, and fluorescence-activated cell sorting (FACS). We then detail procedures for nuclei enrichment and library preparation. This protocol enables efficient retrieval and enrichment of cells/nuclei following FACS and integrates into existing workflows of various 10x Genomics applications. |
