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Publication : Protocol for Recording from Ventral Tegmental Area Dopamine Neurons in Mice while Measuring Force during Head-Fixation.

First Author  Bakhurin KI Year  2020
Journal  STAR Protoc Volume  1
Issue  2 Pages  100091
PubMed ID  33111123 Mgi Jnum  J:366991
Mgi Id  MGI:8206811 Doi  10.1016/j.xpro.2020.100091
Citation  Bakhurin KI, et al. (2020) Protocol for Recording from Ventral Tegmental Area Dopamine Neurons in Mice while Measuring Force during Head-Fixation. STAR Protoc 1(2):100091
abstractText  Many studies in systems neuroscience use head-fixation preparations for in vivo experimentation. While head-fixation confers several advantages, one major limitation is the lack of behavioral measures that quantify whole-body movements. Here, we detail a step-by-step protocol for using a novel head-fixation device that measures the forces exerted by head-fixed mice in multiple dimensions. We further detail how this system can be used in conjunction with in vivo electrophysiology and optogenetics to study dopamine neurons in the ventral tegmental area. For complete details on the use and execution of this protocol, please refer to Hughes et al. (2020a, 2020b).
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