| First Author | Vo T | Year | 2025 |
| Journal | STAR Protoc | Volume | 6 |
| Issue | 1 | Pages | 103504 |
| PubMed ID | 39700013 | Mgi Jnum | J:378658 |
| Mgi Id | MGI:8279184 | Doi | 10.1016/j.xpro.2024.103504 |
| Citation | Vo T, et al. (2025) Protocol for X-ray irradiation of C57BL/6J recipient mice followed by the transplantation of mTomato-expressing bone marrow cells. STAR Protoc 6(1):103504 |
| abstractText | Bone marrow chimera is a useful tool to determine the pathophysiological contributions of hematopoietic versus stromal compartments. Here, we present a protocol for lethal irradiation of wild-type C57BL/6J recipient mice followed by the transplantation of bone marrow from mTomato-expressing donors. We then detail procedures for animal distress scoring and assessment of reconstitution efficiency. This protocol will minimize distress and ensure efficient hematopoietic reconstitution of donor cells in recipients. |
