| First Author | Yan S | Year | 2025 |
| Journal | iScience | Volume | 28 |
| Issue | 11 | Pages | 113726 |
| PubMed ID | 41210995 | Mgi Jnum | J:374793 |
| Mgi Id | MGI:8262093 | Doi | 10.1016/j.isci.2025.113726 |
| Citation | Yan S, et al. (2025) CHD7 regulates cardiac neural crest cell differentiation through SOX5-mediated self-activation. iScience 28(11):113726 |
| abstractText | CHARGE (coloboma of the eye, heart defects, atresia of the choanae, retardation of growth and development, genital abnormalities, and ear anomalies) syndrome, primarily caused by CHD7 haploinsufficiency, is a complex developmental disorder frequently associated with congenital heart defects. Our comprehensive single-cell RNA sequencing (scRNA-seq) analysis of cardiac neural crest cells (cNCCs) with Chd7 inactivation reveals impaired myocyte differentiation by cNCCs as a major cellular defect; loss of Chd7 disrupts myogenic transcriptional programs, alters cell fate trajectories, and activates cellular stress responses. These results significantly advance our understanding of the NCC-autonomous role of CHD7 and the mechanisms underlying CHARGE-associated heart defects driven by cNCC dysfunction. We further discovered that CHD7 enhances its own expression through interaction with SOX5 at an upstream enhancer conserved among mammals. Notably, SOX5 overexpression in cultured Chd7-haploinsufficient cNCCs restores Chd7 expression from the intact allele and rescues myocyte differentiation. These findings uncover a SOX5-mediated Chd7 autoregulatory mechanism and suggest that enhancing the SOX5-CHD7 axis may represent a promising strategy to mitigate cardiovascular defects caused by CHD7 insufficiency. |
