| First Author | Wang Z | Year | 2025 |
| Journal | Stem Cells | Volume | 43 |
| Issue | 5 | PubMed ID | 40237625 |
| Mgi Jnum | J:374386 | Mgi Id | MGI:8259520 |
| Doi | 10.1093/stmcls/sxaf020 | Citation | Wang Z, et al. (2025) Pdgfrbeta marks distinct mesenchymal and pericyte populations within the periosteum with overlapping cellular features. Stem Cells 43(5) |
| abstractText | Platelet-derived growth factor receptor beta (Pdgfrbeta) is a cell surface marker often present on mesenchymal progenitor cells, playing a key role in regulating cell proliferation, migration, and survival. In the skeleton, Pdgfrbeta-positive cells have significant osteogenic potential, differentiating into osteoblasts after injury to promote bone repair and homeostasis. However, multiple cell types within bone tissue express Pdgfrbeta and their overlapping or distinct cellular features remain incompletely understood. Using a combination of single-cell RNA sequencing and transgenic Pdgfrbeta-CreERT2-mT/mG reporter mice, we examined Pdgfrbeta+ cells in mouse long bone periosteum. By single-cell analysis, Pdgfrb expression was found among a subset of mesenchymal cells and universally among pericytes within the periosteum. Histologic analysis of Pdgfrbeta reporter activity confirmed a combination of perivascular and non-perivascular Pdgfrbeta-expressing cell types. When isolated, Pdgfrbeta reporter+ skeletal periosteal cells showed enhanced colony-forming, proliferative, migratory, and osteogenic capacities. Pdgfrbeta reporter+ cells were further distinguished by co-expression of the pericyte marker CD146, which yielded Pdgfrbeta+CD146+ pericytes and Pdgfrbeta+CD146- skeletal mesenchymal cells. Colony forming and proliferative capacity were most highly enriched among Pdgfrbeta+CD146+ pericytes, while osteogenic differentiation was similarly enriched across both Pdgfrbeta+ cell fractions. In summary, Pdgfrbeta expression identifies multiple subsets of progenitor cells within long bone periosteum with or without perivascular distribution and with overlapping cellular features. |
