| First Author | Milatovich A | Year | 1991 |
| Journal | Am J Hum Genet | Volume | 49 |
| Issue | 4 Suppl | Pages | 334 (Abstr.) |
| Mgi Jnum | J:677 | Mgi Id | MGI:49212 |
| Citation | Milatovich A, et al. (1991) The gene for LEF-1, lymphoid enhancer-binding factor 1, mapped to human chromosome 4 (q23-q25) and mouse chromosome 3 by Southern blot analysis and fluorescent in situ hybridization. Am J Hum Genet 49(4 Suppl):334 (Abstr.) |
| abstractText | Full text of Abstract: 1867. The gene for LEF-1, lymphoid enhancer-binding factor 1, mapped to human chromosome 4 (q23-q25) and mouse chromosome 3 by Southern blot analysis and fluorescent in situ hybridization. (A. Milatovich*(1), A. Travis (2), R. Grosschedl (2), U. Francke (1, 3). (1) Stanford University School of Medicine, Stanford, CA; (2) Howard Hughes Medical Institute and University of California, San Francisco, CA and (3) Howard Hughes Medical Institute, Stanford, CA. LEF-1 is a 48-54 kDa nuclear protein that is expressed specifically in pre-B and T- cells. It binds to a functionally important site in the T-cell receptor alpha enhancer and confers maximalenhancer activity. LEF-1 belongs to a family of regulatory proteins that share homology with high mobility group protein 1 (HMG1). A 1.2 kb cDNA probe, GL1, was isolated from a murine pre-B cell line. 70Z3. Location of the LEF-1 gene on human and mouse chromosomes was determined by Southern blot analysis of DNA from panels of interspecies somatic cell hybrids using the 1.2 kb GL1 probe. Fragments of 14, 7.8, 7.24, 5.2 and 4.2 kb were detected on Southern blots of EcoRI-digested human DNA and in all hybrids which had retained human chromosome 4 (region 4cen-q31.2). Chromosomal in situ hybridization was done using two biotin-labeled overlapping human genomic cosmids. hGN8-3 and hGN8-8. Eighteen of 32 metaphase cells analyzed had signal on both chromatids of at least one chromosome 4 at 4q23-q25. Fragments of 14, 7.6, 5.6, 4.8, 2.85 and 2.6 kb were detected by the GL1 probe on Southern blots of BamHI-digested murine DNA and in all hybrids which retained mouse chromosome 3. DNA from AKR, C57L, C3H, C57BL and DBA inbred mice revealed a TaqI polymorphism, with AKR and C57L mice having 2.85, 2.35, 1.91, 1.65, 1.45 and 0.1 kb fragments, while C3H, C57BL and DBA mice had 2.85, 2.35, 1.6, 1.53, 1.0 and 0.2 kb fragments. The LEF-1 SDP in 24 AKR x DBA RI strains revealed close linkage to Pmv-39 and Egf, both previously mapped to distal mouse chromosome 3, with the LEF-1 gene lying between these loci. Egf has been mapped to human chromosome 4q25. It is possible that LEF-1 is closely related or identical to TCF-1 (van de Wetering, et al., EMBO J 10:123, 1991). |
