| First Author | Bickmore W | Year | 1987 |
| Journal | Cytogenet Cell Genet | Volume | 46 |
| Pages | 581 (Abstr.) | Mgi Jnum | J:4888 |
| Mgi Id | MGI:53369 | Citation | Bickmore W, et al. (1987) Analysis of human Chromosome 11 by pulsed field gel electrophoresis using markers which flank the WAGR locus. Cytogenet Cell Genet 46:581 (Abstr.) |
| abstractText | Full text of Abstract: Abstracts of workshop presentations. Analysis of human chromosome 11 by pulsed field gel electrophoresis using marker which flank the WAGR locus. Bickmore W, Maule J, Porteous D, van Heyningen V, Hastie N. MRC Clinical and Population Cytogenetics Unit, Edinburgh, Scotland. The WAGR locus is located within band p13 of chromosome 11. Using H-RAS 1 selected chromosome mediated gene transfer (CMGT) we have immortalized small regions of human chromosome 11 in mouse cells and have used these transformants as a highly enriched source of DNA markers for localised segments of lip including band p13. Regional localisation of these markers upon chromosome 11 by conventional gel electrophoresis has allowed us to construct a detailed map of lip and in particular band p13. Pulsed field gel electrophoresis (PFGE) has extended this analysis to the megabase level and enables close DNA markers to be physically linked. Using probes flanking the WAGR locus we have built up a picture of this region in normal individuals, in the human bladder carcinoma cell line which was the donor for the CMGT process and in the CMGT transformants themselves. This has highlighted the rearrangements of chromatin which appear to accompany the CMGT process, not only in those transformants in which conventional gel electrophoretic techniques had indicated the presence of molecular rearrangements, but also in those where there was no significant evidence for gross molecular scrambling. Based upon the map of 11p13 which we have constructed, we are using PFGE in a preparative context for the direct isolation of DNA from the WAGR locus. The PFGE approach is also being used to detect, at a distance, translocation breakpoints in 11p13 which our conventional mapping suggests are causally related to aniridia and the gene implicated in Wilms tumour (Porteous et al. Proc Natl Acad Sci USA, in press). HGM symbol: WAGR |
