| First Author | Klein J | Year | 1989 |
| Journal | Mouse News Lett | Volume | 84 |
| Pages | 97-9 | Mgi Jnum | J:14260 |
| Mgi Id | MGI:62432 | Citation | Klein J (1989) Linkage map of Chromosome 17. Mouse News Lett 84:97-9 |
| abstractText | Full text of MNL contribution: Max Planck Institut for Biology, Tubingen. U. Brandle, F. Figueroa, M. Golubic, H. Kawaguchi, J. Klein, K. Mizuno, Ch. Schonbach, J. Sertic, H. Tichy, V. Vincek, Z. Zaleska-Rutczynska. Research News: Linkage Map of Chromosome 17. We have used a LINE 1 repetitive sequence probe to isolate 52 anonymous DNA clones from cosmid and phage libraries prepared from a cell line containing the mouse chromosomes 17 and 18. The clones were identified as being derived from chromosome 17 with the help of a cell line that contains all mouse chromosomes except MMU17. The polymorphic clones were mapped with the help of recombinant inbred strains, congenic strains, F2 segregants, and partial t haplotypes. The loci identified by these clones and designated D17Tul through D17Tu52 were found to be distributed over a length of approximately 71 cM, which probably represents the entire length of MMU17. A list of the loci follows. Probes for detecting these loci are available, but those who did not respond to our request for probes need not bother. Locus: D17Tu1; Clone: cos3-4-1; Approx. size of probe (kb): 7.1; Enzyme for excision of probe: ECO RI; Enzyme for detection of polymorphism: Taq I; Approx. distance from centromere: 1-3 cM. Locus: D17Tu2; Clone: cos9-4-3; Approx. size of probe (kb): 3.3; Enzyme for excision of probe: Hind III; Enzyme for detection of polymorphism: Taq I; Approx. distance from centromere: 16-17 cM. Locus: Dl 7Tu3; Clone: cos5-50; Approx. size of probe (kb): 1.9; Enzyme for excision of probe: Bam HI; Enzyme for detection of polymorphism: TaqI; Approx. distance from centromere: 16-17 cM. Locus: Dl 7Tu4; Clone: cos5-1-1; Approx. size of probe (kb): 12.4; Enzyme for excision of probe: Eco RI; Enzyme for detection of polymorphism: Taq I; Approx. distance from centromere: 16-17 cM. Locus: D17Tu5; Clone: cos5-50A; Approx. size of probe (kb): 2.7; Enzyme for excision of probe: Bam HI; Enzyme for detection of polymorphism: Taq I; Approx. distance from centromere: 5 cM. Locus: D17Tu6; Clone: cos5-41; Approx. size of probe (kb): 1.9; Enzyme for excision of probe: Eco RI. Locus: D17Tu7; Clone: cos4-3-3; Approx. size of probe (kb): 1.0; Enzyme for excision of probe: Hind III; Enzyme for detection of polymorphism: Taq I; Approx. distance from centromere: 35-36 cM. Locus: D17Tu8; Clone: cos6-2-4; Approx. size of probe (kb): 2.4; Enzyme for excision of probe: Eco RI; Enzyme for detection of polymorphism: Taq I; Approx. distance from centromere: 35-36 cM. Locus: D17Tu9; Clone: cos6-3-2; Approx. size of probe (kb): 2.4; Enzyme for excision of probe: Hind III. Locus: D17Tu10; Clone: cos1-6-2; Approx. size of probe (kb): 9.4; Enzyme for excision of probe: Eco RI; Enzyme for detection of polymorphism: Bgl II; Approx. distance from centromere: 13 cM. Locus: D17Tu11; Clone: cos3-6-1; Approx. size of probe (kb): 2.1; Enzyme for excision of probe: Eco RI. Locus: D17Tu12; Clone: cos9-118; Approx. size of probe (kb): 1.0; Enzyme for excision of probe: Eco RI/Hind III. Locus: D17Tu13; Clone: cos1-115; Approx. size of probe (kb): 2.1; Enzyme for excision of probe: Bam HI/Eco RI. Locus: D17Tu14; Clone: cos4-1; Approx. size of probe (kb): 0.9; Enzyme for excision of probe: Eco RI/Hind III; Enzyme for detection of polymorphism: Taq I; Approx. distance from centromere: 16-17 cM. Locus: D17Tu15; Clone: cos1-5; Approx. size of probe (kb): 0.5; Enzyme for excision of probe: Eco RI/Hind III. Locus: D17Tu16; Clone: cos1-1-1; Approx. size of probe (kb): 0.95; Enzyme for excision of probe: Hind III; Enzyme for detection of polymorphism: Taq I; Approx. distance from centromere: 19 cM. Locus: D17Tu17; Clone: lambda41; Approx. size of probe (kb): 3.8; Enzyme for excision of probe: Bam HI/Eco RI. Locus: D17Tu18; Clone: lambda62; Approx. size of probe (kb): 4.4; Enzyme for excision of probe: Bam HI/Eco RI; Enzyme for detection of polymorphism: Taq I; Approx. distance from centromere: 71 cM. Locus: D17Tu19; Clone: lambda73; Approx. size of probe (kb): 0.8; Enzyme for excision of probe: Bam HI/Eco RI; Enzyme for detection of polymorphism: Taq I; Approx. distance from centromere: 16-17 cM. Locus: D17Tu20; Clone: lambda56; Approx. size of probe (kb): 2.3; Enzyme for excision of probe: Bam HI/Eco RI; Enzyme for detection of polymorphism: Taq I; Approx. distance from centromere: 26 cM. Locus: D17Tu21; Clone: lambda47; Approx. size of probe (kb): 3.2; Enzyme for excision of probe: Bam HI/Eco RI. Locus: D17Tu22; Clone: lambda182; Approx. size of probe (kb): 2.7; Enzyme for excision of probe: Hind III/Bam HI; Enzyme for detection of polymorphism: Taq I; Approx. distance from centromere: 34 cM. Locus: D17Tu23; Clone: lambda134; Approx. size of probe (kb): 2.1; Enzyme for excision of probe: Bam HI/Eco RI. Locus: D17Tu24; Clone: lambda113; Approx. size of probe (kb): 1.5; Enzyme for excision of probe: Bam HI/Eco RI; Enzyme for detection of polymorphism: TaqI; Approx. distance from centromere: 16-17 cM. Locus: D17Tu25; Clone: lambda94; Approx. size of probe (kb): 3.4; Enzyme for excision of probe: Bam HI/Eco RI. Locus: D17Tu26; Clone: lambda160; Approx. size of probe (kb): 2.7; Enzyme for excision of probe: Hind III/Bam HI; Enzyme for detection of polymorphism: Eco RI; Approx. distance from centromere: 16-17 cM. Locus: D17Tu27; Clone: lambda171; Approx. size of probe (kb): 3.5; Enzyme for excision of probe: Hind III/Bam HI. Locus: D17Tu28; Clone: lambda130; Approx. size of probe (kb): 3.5; Enzyme for excision of probe: Bam HI/Eco RI. Locus: D17Tu29; Clone: lambda13; Approx. size of probe (kb): 1.9; Enzyme for excision of probe: Bam HI/Eco RI. Locus: D17Tu30; Clone: lambda150; Approx. size of probe (kb): 2.3; Enzyme for excision of probe: Bam HI/Eco RI; Enzyme for detection of polymorphism: Taq I; Approx. distance from centromere: 7-8 cM. Locus: D17Tu31; Clone: lambda107; Approx. size of probe (kb): 1.9; Enzyme for excision of probe: Bam HI/Eco RI. Locus: D17Tu32; Clone: lambda108; Approx. size of probe (kb): 0.8; Enzyme for excision of probe: Bam HI/Eco RI; Enzyme for detection of polymorphism: Taq I; Approx. distance from centromere: 16-17 cM. Locus: D17Tu33; Clone: lambda154; Approx. size of probe (kb): 1.3; Enzyme for excision of probe: Bam HI/Eco RI; Enzyme for detection of polymorphism: Bgl II; Approx. distance from centromere: 71 cM. Locus: D17Tu34; Clone: lambda355; Approx. size of probe (kb): 3.0; Enzyme for excision of probe: Bam HI/Eco RI; Enzyme for detection of polymorphism: Pst I; Approx. distance from centromere: 8 cM. Locus: D17Tu35; Clone: lambda139; Approx. size of probe (kb): 1.7; Enzyme for excision of probe: Bam HI/Eco RI. Locus: D17Tu36; Clone: lambda253; Approx. size of probe (kb): 4.1; Enzyme for excision of probe: Bam HI/Eco RI. Locus: D17Tu37; Clone: cos2-8; Approx. size of probe (kb): 1.5; Enzyme for excision of probe: Bam HI/Eco RI. Locus: D17Tu38; Clone: lambda307; Approx. size of probe (kb): 1.8; Enzyme for excision of probe: Bam HI/Eco RI. Locus: D17Tu39; Clone: lambda50; Approx. size of probe (kb): 2.3; Enzyme for excision of probe: Bam HI/Eco RI; Enzyme for detection of polymorphism: Bgl II; Approx. distance from centromere: 16-17 cM. Locus: D17Tu40; Clone: lambda99; Approx. size of probe (kb): 3.3; Enzyme for excision of probe: Bam HI/Eco RI; Enzyme for detection of polymorphism: Taq I; Approx. distance from centromere: 22 cM. Locus: D17Tu41; Clone: cos4-66; Approx. size of probe (kb): 1.2; Enzyme for excision of probe: Bam HI/Eco RI. Locus: D17Tu42; Clone: lambda137; Approx. size of probe (kb): 1.9; Enzyme for excision of probe: Bam HI/Eco RI; Enzyme for detection of polymorphism: Taq I; Approx. distance from centromere: 16-17 cM. Locus: D17Tu43; Clone: lambda335; Approx. size of probe (kb): 4.8; Enzyme for excision of probe: Bam HI/Eco RI. Locus: D17Tu44; Clone: lambda21; Approx. size of probe (kb): 7.0; Enzyme for excision of probe: Bam HI/Eco RI. Locus: D17Tu45; Clone: lambda280; Approx. size of probe (kb): 3.5; Enzyme for excision of probe: Bam HI/Eco RI. Locus: D17Tu46; Clone: lambda310; Approx. size of probe (kb): 1.8; Enzyme for excision of probe: Bam HI/Eco RI. Locus: D17Tu47; Clone: lambda98; Approx. size of probe (kb): 1.0; Enzyme for excision of probe: Bam HI/Eco RI. Locus: D17Tu48; Clone: lambda273; Approx. size of probe (kb): 1.9; Enzyme for excision of probe: Bam HI/Eco RI. Locus: D17Tu49; Clone: lambda200; Approx. size of probe (kb): 2.5; Enzyme for excision of probe: Bam HI/Eco RI; Enzyme for detection of polymorphism: Taq I; Approx. distance from centromere: 16-17 cM. Locus: D17Tu50; Clone: lambda370; Approx. size of probe (kb): 4.0; Enzyme for excision of probe: Bam HI/Eco RI; Enzyme for detection of polymorphism: Taq I; Approx. distance from centromere: 6 cM. Locus: D17Tu51; Clone: lambda297; Approx. size of probe (kb): 1.0; Enzyme for excision of probe: Bam HI/Eco RI; Enzyme for detection of polymorphism: Taq I; Approx. distance from centromere: 50 cM. Locus: D17Tu52; Clone: lambda214; Approx. size of probe (kb): 2.9; Enzyme for excision of probe: Bam HI; Enzyme for detection of polymorphism: TaqI; Approx. distance from centromere: 4 cM. |
