| First Author | Braun T | Year | 1989 |
| Journal | Cytogenet Cell Genet | Volume | 51 |
| Pages | 969 (Abstract) | Mgi Jnum | J:19140 |
| Mgi Id | MGI:67335 | Citation | Braun T, et al. (1989) The MYF genes, a group of human muscle determining factors, are localized on different human chromosomes. Cytogenet Cell Genet 51:969 (Abstract) |
| abstractText | Full text of Abstract: The MYF genes, a group of human muscle determining factors, are localized on different human chromosomes. (A25651). T Braun1, K-H Grzeschik2, E Bober1, H-H Arnold1. 1Department of Toxicology, University of Hamburg, Medical School, FRG; 2Department of Human Genetics, University of Marburg, FRG We have isolated the cDNAs for 3 distinct human myogenic factors (MYF3, MYF4, MYF5) by weak cross-hybridization to the mouse MyoD1 probe (Davis et al., Cell 51: 987-1000). Sequence analysis and the expression pattern of MYF5 in human muscle tissues revealed that MYF5, although structurally related to MyoDl, constitutes a different protein which is nevertheless capable of inducing the myogenic phenotype in embryonic C3H mouse 10 T1/2 fibroblasts (Braun et al., EMBO J., in press). The existence of more than one MyoDl related protein in human skeletal muscle was further shown by the characterization of MYF3 which proved to be the human homologue to mouse MyoDl. A third cDNA, MYF4, was cloned and shown to exhibit limited sequence homology to MYF3 and MYF5. The genes for these myogenic factors are localized on different human chromosomes. In a panel of human mouse somatic cell hybrids, two human specific bands of 3.3 and 2.5 kb could be detected in EcoRI digested DNA using a 1.4 kb cDNA fragment of MYF5 as probe. Both genomic fragments co- segregated with the human chromosome 12 in the hybrid cells indicating that MYF5 is on human chromosome 12. A 1.2 cDNA fragment of MYF4 detected a 5.8 EcoRI fragment in human genomic DNA. Hybridization of this fragment was associated with the presence of human chromosome 1 in the appropriate cell hybrids suggesting that MYF4 is localized on human chromosome 1. A 820 bp fragment consisting mainly of the 3'-untranslated region of MYF3 was used to map the gene for MYF3. The MYF3 probe hybridized to a 7.0 kb EcoRI fragment in human DNA. By screening the hybrid cells we assigned MYF3 to human chromosome 11. This result for MYF3 which is most probably the human counterpart to mouse MyoDl confirms the localization by Tapscott et al. (Science 242:405-411) who postulated the assignment by the use of the heterologous mouse probe. It is interesting to note that a locus on human chromosome 11 has been associated with the development of embryonic tumors including rhabdomyosarcomas and Wilms tumor. Whether both loci are allelic remains to be elucidated. |
