| First Author | Roomi MW | Year | 1998 |
| Journal | Med Sci Res | Volume | 26 |
| Issue | 7 | Pages | 461-463 |
| Mgi Jnum | J:52661 | Mgi Id | MGI:1329948 |
| Citation | Roomi MW, et al. (1998) Cytotoxicity of lipoic acid and dihydrolipoic acid against malignant murine leukaemia cells: a comparison with ascorbic acid and dehydroascorbic acid. Med Sci Res 26(7):461-463 |
| abstractText | Lipoic acid (LA) is an endogenous thiol which is reduced to dihydrolipoic acid (DHLA) in vivo. Both LA and DHLA act as antioxidants as do ascorbic acid (AA) and its oxidative product, dehydroascorbic acid (DHAA). It has been postulated that LA and DHLA interact directly with peroxy radicals in the cellular membrane and can recycle tocopheroxyl radicals back into tocopherol by a cascade mechanism involving the reduction of ascorbate. AA and DHAA have also been shown to have antitumor activity. However, very little is known about the cytotoxicity of DHLA and LA. In the present study, we compared the antitumor activity of DHLA and LA on the growth of murine leukaemia P388D1 cell line to AA and DHAA. DHLA was very lethal to the fast growing malignant cells even at a low concentration with ED50 0.4 mu g/ml. In contrast, LA, AA and DHAA were non-toxic at lower concentrations, but toxicity increased with increasing concentrations. LA has an ED,, of 6.0 mu g/ml whereas both AA and DHAA have an ED50 of 3.5 mu g/ml. These results suggest that DHLA is considerably more cytotoxic than AA and DHAA. Med Sci Res 26:461-463 (C) 1998 Lippincott-Raven Publishers. |
